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KMID : 0613820130230020299
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Journal of Life Science
2013 Volume.23 No. 2 p.299 ~ p.305
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Effect of Glucose on Swarming Motility of Paenibacillus sp. CK214
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Kang Sung-Wan
Yoo Ah-Young
Kang Ho-Young
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Abstract
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Paenibacillus is a gram-positive, spore-forming aerobes that was previously classified as a Bacillus species. Paenibacillus sp. CK214 was highly motile on LB agar plates and showed typical colonial morphology of Paenibacillus. However, its motility was defective in the absence of glucose. Electron microscopic observation revealed that the cells of CK214 cultured on LB agar plates were peritrichously flagellated but not flagellated in the presence of glucose. Flagellar filaments were purified by centrifugation after shearing off from the CK214 cells with vigorous pipetting. The purified protein was composed of a single flagellin with an apparent molecular size of 29 kDa. Recognition of the protein by anti-Edwardsiella tarda flagellin protein antibody demonstrates that the protein is a flagellin protein. A decreased level of flagellin protein was detected in CK214 cells grown under glucose-supplemented media.
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KEYWORD
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Flagellin, glucose effect, Paenibacillus, swarming
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